速尿对豚鼠离体胃黏膜胃酸分泌的抑制作用。

Suppression of gastric acid secretion by furosemide in isolated gastric mucosa of guinea pig.

作者信息

Ayalon A, Corcia A, Klemperer G, Caplan S R

出版信息

Am J Physiol. 1980 Dec;239(6):G532-5. doi: 10.1152/ajpgi.1980.239.6.G532.

DOI:10.1152/ajpgi.1980.239.6.G532

Abstract

The effect of furosemide on acid secretion and Cl- transport was studied in isolated fundic mucosa of the guinea pig. Furosemide (10(-3) M), applied to the serosal side produced an immediate effect on the short-circuit current (Isc), lowering it by 47 +/- 2%. Potential difference decreased by 29 +/- 3%, electrical conductance by 18 +/- 4%, acid secretion by 38 +/- 1%, and net flux of Cl- from serosal-to-mucosal side by 37%. Application of the drug to the mucosal side produced similar effects on acid secretion and on the electrical parameters. It is suggested that furosemide blocks the entrance of Cl-, by the Na+--Cl- cotransport mechanism, through the basolateral membrane of the secreting cell. The consequent reduction in electrogenic Cl- transport would cause Isc and acid secretion to decrease. A reduction of Cl- conductance of the apical membrane, upon mucosal application of the drug, would cause similar effects on acid secretion and Cl- transport.

摘要

在豚鼠离体胃底黏膜中研究了呋塞米对酸分泌和氯离子转运的影响。将呋塞米（10⁻³ M）施加于浆膜侧，对短路电流（Isc）产生即刻效应，使其降低47±2%。电位差降低29±3%，电导降低18±4%，酸分泌降低38±1%，氯离子从浆膜侧向黏膜侧的净通量降低37%。将该药物施加于黏膜侧，对酸分泌和电参数产生类似效应。提示呋塞米通过钠-氯共转运机制，阻断氯离子通过分泌细胞基底外侧膜的进入。由此导致的电生性氯离子转运减少会使Isc和酸分泌降低。在黏膜侧施加该药物时，顶端膜氯离子电导降低，会对酸分泌和氯离子转运产生类似效应。