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Determination of enzyme kinetic parameters by continuous addition of substrate to a single reaction mixture and analysis by a tangent-slope procedure. II. Application of the method to soluble and immobilized enzymes.

作者信息

LeBlond D J, Ashendel C L, Wood W A

出版信息

Anal Biochem. 1980 May 15;104(2):370-85. doi: 10.1016/0003-2697(80)90088-3.

DOI:10.1016/0003-2697(80)90088-3
PMID:7446963
Abstract
摘要

相似文献

1
Determination of enzyme kinetic parameters by continuous addition of substrate to a single reaction mixture and analysis by a tangent-slope procedure. II. Application of the method to soluble and immobilized enzymes.通过向单一反应混合物中连续添加底物并采用切线斜率法进行分析来测定酶动力学参数。II. 该方法在可溶性酶和固定化酶中的应用。
Anal Biochem. 1980 May 15;104(2):370-85. doi: 10.1016/0003-2697(80)90088-3.
2
Determination of enzyme kinetic parameters by continuous addition of substrate to a single reaction mixture and analysis by a tangent-slope procedure. I. Analysis of the method using computed progress curves.
Anal Biochem. 1980 May 15;104(2):355-69. doi: 10.1016/0003-2697(80)90087-1.
3
The applications of immobilized enzymes in automated analysis.固定化酶在自动化分析中的应用。
Methods Enzymol. 1976;44:633-46. doi: 10.1016/s0076-6879(76)44044-2.
4
On-line enzymatic amplification by substrate cycling in a dual bioreactor with rotation and amperometric detection.
Anal Biochem. 1994 Aug 1;220(2):297-302. doi: 10.1006/abio.1994.1340.
5
Properties of lactate dehydrogenase immobilized in a lipid-protein matrix.固定在脂蛋白基质中的乳酸脱氢酶的性质。
FEBS Lett. 1976 Dec 15;72(1):93-7. doi: 10.1016/0014-5793(76)80820-4.
6
Bienzymatic Sequential Reaction on Microgel Particles and Their Cofactor Dependent Applications.微凝胶颗粒上的双酶顺序反应及其辅酶依赖性应用
Biomacromolecules. 2016 May 9;17(5):1610-20. doi: 10.1021/acs.biomac.5b01745. Epub 2016 Apr 8.
7
[Regulation of enzyme activity in adsorptive enzyme systems. II. Influence of dextran sulfate on catalytic properties of lactate dehydrogenase (isozyme M4)].[吸附酶系统中酶活性的调节。II. 硫酸葡聚糖对乳酸脱氢酶（同工酶M4）催化特性的影响]
Mol Biol (Mosk). 1979 May-Jun;13(3):559-66.
8
Ceramic membrane microfilter as an immobilized enzyme reactor.
Enzyme Microb Technol. 1992 Oct;14(10):813-8. doi: 10.1016/0141-0229(92)90097-8.
9
[Regulation of enzyme activity in adsorptive enzyme systems. III. Interaction of pig muscle lactate dehydrogenase with F-actin].[吸附酶系统中酶活性的调节。III. 猪肌肉乳酸脱氢酶与F-肌动蛋白的相互作用]
Mol Biol (Mosk). 1983 Mar-Apr;17(2):430-6.
10
Quantitative analysis of protein: immobilized dye interaction.蛋白质的定量分析：固定化染料相互作用
J Biol Chem. 1984 Mar 25;259(6):3796-9.

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Enzymes of carnitine acylation. Is overt carnitine palmitoyltransferase of liver peroxisomal carnitine octanoyltransferase?肉碱酰化酶。肝脏过氧化物酶体肉碱辛酰转移酶的明显肉碱棕榈酰转移酶是吗？
Biochem J. 1988 Jan 1;249(1):231-7. doi: 10.1042/bj2490231.