Determination of enzyme kinetic parameters by continuous addition of substrate to a single reaction mixture and analysis by a tangent-slope procedure. I. Analysis of the method using computed progress curves.
作者信息
LeBlond D J, Ashendel C L, Wood W A
出版信息
Anal Biochem. 1980 May 15;104(2):355-69. doi: 10.1016/0003-2697(80)90087-1.