Alykov N M
Antibiotiki. 1980 Nov;25(11):831-4.
Azo-derivatives of chromotropic acid and azo-dyes of the stilbene series were studies as possible reagents for photometric determination of aminoglycoside antibiotics (AB) in biological materials. The complex of Pr3+ with stilbazochrome-P (Sch-P) proved to be the best precipitant of AB. P (Sch-P) with Pr3+ and AB in acetate and citrate buffer-solutions formed a precipitate with AB:Pr:Sch-P = 1 : 1 : 3. Most of the metallic cations and anions did not prevent formation of the precipitate in the citrate buffer. A procedure for determination of 0.01--100 micrograms of AB in biological materials was developed with the use of Sch-P. The procedure is based on separation of the AB:Pr:Sch-P precipitate and photometry of the sypernatant solution at 560 nm.
研究了变色酸偶氮衍生物和芪系偶氮染料作为生物材料中氨基糖苷类抗生素（AB）光度测定的可能试剂。Pr3+与芪偶氮铬红-P（Sch-P）的络合物被证明是AB的最佳沉淀剂。在乙酸盐和柠檬酸盐缓冲溶液中，Pr3+、AB与Sch-P形成了AB:Pr:Sch-P = 1 : 1 : 3的沉淀。大多数金属阳离子和阴离子不会阻止在柠檬酸盐缓冲溶液中形成沉淀。利用Sch-P建立了生物材料中0.01--100微克AB的测定方法。该方法基于AB:Pr:Sch-P沉淀的分离以及上清液在560nm处的光度测定。
