Comparative investigations of in vitro and in vivo scalding of mouse skin.

Mouse skin was scalded in vitro by the wet (immersion) method and dry (metal stamp) method. For in vivo scalding only the dry method was used. The content of dry mass of skin was investigated. In extracts of scalded skin the content of soluble components: protein, collagen, albumin and IgG was determined. The analyses and statistic estimation revealed that with respect to the observed effects the in vivo scalding of skin is similar to the ion vitro dry scalding but different from the in vitro wet method. Moreover, it is suggested that protein substances which are products of thermic denaturation of skin can penetrate from scalded skin into the surrounding tissues.