[Proliferative characteristics of human peripheral blood leukocytes cultured with different cryoprotectors].

The effect of cryoprotectors, both penetrating (glycerol) and nonpenetrating (polyethyleneoxide 400, oxyethylated glycerol) into cells, used in different concentrations in a short-term culture of human peripheral blood was studied in connection with the proliferating activity of leucocytes. A similar pattern of the effect of these substances on the cell growth in culture was observed. Some features of leucocyte proliferation were established such as a decrease of the mitotic index, an increase in the relative number of metaphases and the number of pathological mitoses with the increase in the concentration of the cryoprotectors under study. Some cytopathic changes in the cells were found.