气液色谱法在腈水解酶和酰胺酶研究中的应用（作者译）

[Use of gas-liquid chromatography for the study of nitrilases and amidases (author's transl)].

作者信息

Jallageas J C, Arnaud A, Galzy P

出版信息

J Chromatogr. 1978 Dec 1;166(1):181-7. doi: 10.1016/s0021-9673(00)92261-1.

Abstract

A gas-liquid chromatographic procedure is described for the determination of nitrilasic and amidasic activities. This method allows to monitor the kinetics of the hydrolysis of volatile nitriles and amides and because of its sensitivity, to determine the Michaelis constants Km of the acetonitrilase and the acetamidase of Brevibacterium R 312. For these enzymes, a correlation is shown between the kinetics monitored by proton nuclear magnetic resonance spectroscopy and gas-liquid chromatography.

摘要

本文描述了一种用于测定腈水解酶和酰胺酶活性的气-液色谱法。该方法能够监测挥发性腈和酰胺的水解动力学，并且由于其灵敏度，可以测定短杆菌R 312的乙腈水解酶和乙酰胺酶的米氏常数Km。对于这些酶，质子核磁共振光谱法监测的动力学与气-液色谱法之间显示出相关性。