嗜热栖热放线菌中的核糖体抑制因子和抗抑制因子：对放线菌酮的抗性
Ribosomal suppressors and antisuppressors in Podospora anserina: resistance to cycloheximide.
作者信息
Coppin-Raynal E
出版信息
J Bacteriol. 1977 Sep;131(3):876-83. doi: 10.1128/jb.131.3.876-883.1977.
Abstract
Informational suppressors and antisuppressors have been previously isolated in Podospora anserina, and a range of exclusively genetic arguments have led to the assumption that they correspond to ribosomal mutations. An in vivo and in vitro comparison of the effect of the ribosomal inhibitor cycloheximide on wildtype and mutant strains described in this paper confirms the ribosomal hypothesis for at least some mutants. Indeed, the four mutants in the AS3 gene were cycloheximide resistant, and their ribosomes were found to be resistant when analyzed by polyuridyl-directed polyphenylalanine systhesis. On the other hand, ribosomes from two su 1 mutants were hypersensitive to the drug.
摘要
信息性抑制因子和抗抑制因子先前已在嗜热栖热放线菌中分离出来，一系列纯粹基于遗传学的论据导致人们假定它们与核糖体突变相对应。本文对核糖体抑制剂环己酰亚胺对野生型和突变菌株的体内和体外作用进行了比较，证实了至少对某些突变体而言的核糖体假说。的确，AS3基因中的四个突变体对环己酰亚胺具有抗性，并且当通过聚尿苷酸指导的聚苯丙氨酸合成进行分析时，发现它们的核糖体具有抗性。另一方面，来自两个su 1突变体的核糖体对该药物高度敏感。
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