Gremigni D, Vannelli G, Sgaragli G P, Mengazzini I
Boll Soc Ital Biol Sper. 1980 May 30;56(10):1078-82.
12 male Sprague-Dawley rats were given chlorimipramine through gastric sound at a daily dose of 150 mg/Kg body weight for two weeks. From the rats, killed at the end of the treatment, were collected lung fragments, some of which were examined by electron microscopy and others by light microscopy, following particular fixation and colouring procedures. The light microscopy revealed that many alveoli contained numerous cells, large and clear, the cytoplasma of which contained vacuoles of different dimensions, for which these cells appeared from " foam cells". Besides, they reacted positively to the method of Baker and were intensely coloured in dark grey, after fixation with osmium tetroxide-natrium iodide. The ultrastructural examination evidentiated two main types of cells: the first comprised large, round cells, classifiable as alveolar macrophages while the second type comprised cells that were classifiable as type II alveolar cells, in which, apart from important mitochondrial modifications, numerous vacuoles of different sizes were detectable. The AA. consider the alterations of the type II alveolar cells as a characteristic morphological aspect of phospholipidosis from chlorimipramine.
12只雄性Sprague-Dawley大鼠通过胃管给予氯米帕明，每日剂量为150毫克/千克体重，持续两周。在治疗结束时处死大鼠，收集肺组织碎片，其中一些按照特定的固定和染色程序进行电子显微镜检查，另一些进行光学显微镜检查。光学显微镜显示，许多肺泡含有大量大而清晰的细胞，其细胞质含有不同大小的空泡，因此这些细胞看起来像“泡沫细胞”。此外，在用四氧化锇-碘化钠固定后，它们对贝克方法呈阳性反应，并被染成深灰色。超微结构检查显示出两种主要类型的细胞：第一种包括大的圆形细胞，可归类为肺泡巨噬细胞，而第二种类型的细胞可归类为II型肺泡细胞，其中除了重要的线粒体改变外，还可检测到许多不同大小的空泡。作者认为II型肺泡细胞的改变是氯米帕明所致磷脂沉积症的特征性形态学表现。
