麻醉对大鼠脑突触体膜流动性和乙酰胆碱酯酶活性的影响。

Effect of anesthesia on membrane fluidity and acetylcholinesterase activity in rat brain synaptosomes.

作者信息

Zolese G, Curatola G, Mazzanti L, Lenaz G, Pastuszko A

出版信息

Boll Soc Ital Biol Sper. 1980 Jun 15;56(11):1109-13.

Abstract

Anesthesia by means of ketamine induces an increase of fluidity in the hydrophobic area of rat brain synaptic membranes, as proved by the spin label 16-doxyl stearate, in contrast with no change induced in the order parameter of 5-doxylstearate that probes the membrane surface. These effects are qualitatively similar to those observed by ketamine addition in vitro. On the contrary, acetylcholinesterase from synaptic membranes, although inhibited in vitro by ketamine, is activated in ketamine-anesthetised rats suggested that metabolic changes are at the basis of the effects observed in vivo.

摘要

通过氯胺酮进行麻醉会使大鼠脑突触膜疏水区域的流动性增加，这已由自旋标记物16 - 硬脂酰氧基氮氧自由基证明，与之形成对比的是，探测膜表面的5 - 硬脂酰氧基氮氧自由基的序参数没有变化。这些效应在性质上与体外添加氯胺酮时观察到的效应相似。相反，突触膜中的乙酰胆碱酯酶虽然在体外会被氯胺酮抑制，但在氯胺酮麻醉的大鼠中却被激活，这表明代谢变化是体内观察到的这些效应的基础。