Pacini P, Orlandini G E, Holstein A F
Boll Soc Ital Biol Sper. 1980 Apr 30;56(8):781-5.
The human excurrent duct system of the testis (rete testis, ductuli efferentes, ductus epididymis and vas deferens) have been studied by the S.E.M. The specimens have been taken from subjects who underwent surgery for prostatic carcinoma. For the S.E.M. the specimens have been prepared according to usual techniques: fixed with 2.5% glutaraldehyde and 1% OsO4 in Millonig's buffer, dehydrated in ethanol, dried by the critical point technique in CO2 and coated with gold-palladium 10%. The cavities of the rete testis show a flat epithelium with short microvilli and single cilia. The ductuli efferentes are lined by long-ciliated columnar elements and by stubby elements provided with microvilli. Globular extrusions, probably representing the swollen apices of epithelial cells, have been interpretated like the expression of a secretory activity. The ductus epididymis is lined by high columnar epithelium provided with thick microvilli. In the lumen it is possible to observe little globular masses with variable diameters, isolated or more often grape-shaped. These have been interpretated as the expression of secretory activity microapocrine in nature. The vas deferens is bounded with columnar epithelial cells with variable morphology. Some of them, infact, have crowded microvilli, while some others have swollen apices bulging into the lumen. These globular extrusions are probably the expression of the secretory activity.
利用扫描电子显微镜对睾丸的人体输出管道系统（睾丸网、输出小管、附睾管和输精管）进行了研究。标本取自接受前列腺癌手术的患者。对于扫描电子显微镜检查，标本按照常规技术制备：用2.5%戊二醛和1%四氧化锇在米隆尼缓冲液中固定，在乙醇中脱水，通过二氧化碳临界点技术干燥，并用10%的金钯镀膜。睾丸网的腔显示为扁平上皮，有短微绒毛和单个纤毛。输出小管内衬有长纤毛柱状细胞和有微绒毛的粗短细胞。球状突起可能代表上皮细胞肿胀的顶端，被解释为分泌活动的表现。附睾管内衬有高柱状上皮，有粗微绒毛。在管腔内可以观察到直径不一的小球状团块，单个或更多时候呈葡萄状。这些被解释为微顶浆分泌性质的分泌活动的表现。输精管由形态各异的柱状上皮细胞界定。事实上，其中一些细胞有密集的微绒毛，而另一些细胞的顶端肿胀并突入管腔。这些球状突起可能是分泌活动的表现。
