对含脂质噬菌体phi 6的物理测量。
Physical measurements on the lipid-containing bacteriophage phi 6.
作者信息
Berger H, Kennedy K
出版信息
Biochim Biophys Acta. 1980 Nov 17;633(1):68-76. doi: 10.1016/0304-4165(80)90038-0.
Bacteriophage phi 6 has been studied by small-angle X-ray scattering intensity-fluctuation spectroscopy, analytical ultracentrifugation, and spectroscopy. The sedimentation coefficient (S(0)0,w) is 375 S, the diffusion coefficient (D(0)20, w) is 2.66 x 10(-8) cm 2/s. Using the Svedberg equation and and an estimate of the partial specific volume, the Mr is 1.49 +/- 0.32 x 10(8). A simple model which describes phi 6, is a central sphere consisting of RNA and protein of radius 330 A and an outer shell of low electron density 40 A thick. The RNA may form five concentric shells in the region r = 140-290 A.
噬菌体φ6已通过小角X射线散射强度波动光谱法、分析超速离心法和光谱法进行了研究。沉降系数（S(0)0,w）为375 S，扩散系数（D(0)20,w）为2.66×10^(-8) cm²/s。利用斯维德伯格方程和比容估计值，相对分子质量为1.49±0.32×10^8。一个描述φ6的简单模型是，一个由半径为330 Å的RNA和蛋白质组成的中心球体，以及一个厚度为40 Å的低电子密度外壳。RNA可能在r = 140 - 290 Å区域形成五个同心壳层。
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