Eckstein R, Mempel W, Hannig K, Wilmanns W
Blut. 1980 Dec;41(6):459-64. doi: 10.1007/BF01007772.
EMT indicator cells were incubated for 1 h in the supernatant of a 3 h MLC. Their electrophoretic mobility was then measured by an analytical, carrier-free electrophoresis system. The alteration of their mobility against a medium-standard was calculated in per cent and correlated with the conventional measured MLR-cpm. According to our results the correlation of the two quantities is statistically highly significant. This method could enable recognition of a positive or negative MLC after only 4 h. This fact could be of great importance for histocompatibility testing and transplantation.
将 EMT 指示细胞在 3 小时混合淋巴细胞培养（MLC）的上清液中孵育 1 小时。然后通过无载体分析电泳系统测量其电泳迁移率。计算其相对于培养基标准品的迁移率变化百分比，并与传统测量的混合淋巴细胞反应（MLR）每分钟计数（cpm）相关联。根据我们的结果，这两个量之间的相关性在统计学上具有高度显著性。该方法仅需 4 小时就能识别阳性或阴性 MLC。这一事实对于组织相容性检测和移植可能具有重要意义。
