The effect of VP 16-213 on NBT reduction in the normal polymorphonuclear neutrophil.

VP 16-213, an antineoplastic, semisynthetic podophyllotoxin derivative, inhibits NBT reduction in the polymorphonuclear neutrophil (PMN). In the resting PMN an almost complete inhibition is seen at a concentration of 170 microM and a partial inhibition is seen at 17 microM. In PMN's stimulated with zymosan activated serum, partial inhibition is seen at concentrations of both 170 microM and 17 microM. VP 16-213 shows no significant inhibition of NBT reduction in PMN's stimulated by methylene blue. VP 16-213 may alter normal respiratory function in the PMN as reflected by inhibition of NBT reduction.