Rotundo R L, Fambrough D M
Cell. 1980 Nov;22(2 Pt 2):595-602. doi: 10.1016/0092-8674(80)90369-4.
Acetylcholinesterase (AChE) and acetylcholine receptors (AChR) are muscle-specific glycoproteins present (AChR) are muscle-specific glycoproteins present in cultured chick embryo muscle cells. The first is found as both a secreted and a membrane-bound enzyme whereas the ACh receptor is strictly an integral membrane protein. We have studied the transport and externalization of these two proteins in the same cells using several compounds known to affect secretory processes: colchicine, tunicamycin and the ionophores X-537A, Nigericin and Monensin. Under all experimental conditions, any change in the rate of AChE secretion was accompanied by an identical change in the rate of ACh receptor incorporation into the plasma membrane. These studies were designed to test directly the hypothesis that secreted and integral membrane proteins are transported together to the plasma membrane. Our results are consistent with a single transport pathway in muscle cells for the externalization of membrane and secreted proteins.
乙酰胆碱酯酶（AChE）和乙酰胆碱受体（AChR）是存在于培养的鸡胚肌肉细胞中的肌肉特异性糖蛋白。前者既以分泌型酶又以膜结合型酶的形式存在，而ACh受体则严格来说是一种整合膜蛋白。我们使用几种已知会影响分泌过程的化合物：秋水仙碱、衣霉素以及离子载体X-537A、尼日利亚菌素和莫能菌素，在同一细胞中研究了这两种蛋白质的转运和外化。在所有实验条件下，AChE分泌速率的任何变化都伴随着ACh受体掺入质膜速率的相同变化。这些研究旨在直接检验分泌型蛋白和整合膜蛋白一起转运到质膜的假说。我们的结果与肌肉细胞中膜蛋白和分泌蛋白外化的单一转运途径一致。
