两种本斯-琼斯蛋白的免疫化学研究。

An immunochemical study of two Bence-Jones proteins.

作者信息

Wang W P, Chuang C Y, Lin K T, Lee C S, Chen C Y, Chen W Y

出版信息

Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1980 Mar;13(1):44-51.

Abstract

Two Bence-Jones proteins, one of kappa and the other of lambda type, from the urine of two myeloma patients were identified by protein electrophoresis (PEP) and immunoelectrophoresis (IEP). Urine specimens were concentrated and dialyzed with Ultrafiltration. The concentrated products were passed through a Sephadex G-100 column, and the fractions of Bence-Jones proteins were recycled in diethylaminoethyl (DEAE) ion exchange chromatography. Purified Bence-Jones proteins were mixed thoroughly with complete Freund adjuvant and inoculated into rabbits. The monospecific antisera to kappa and lambda chains were obtained by absorption with Bence-Jones proteins of the opposite types.

摘要

通过蛋白质电泳（PEP）和免疫电泳（IEP）鉴定了两名骨髓瘤患者尿液中的两种本周氏蛋白，一种为κ型，另一种为λ型。尿液标本经超滤浓缩和透析。浓缩产物通过葡聚糖G - 100柱，本周氏蛋白组分在二乙氨基乙基（DEAE）离子交换色谱中进行循环。将纯化的本周氏蛋白与完全弗氏佐剂充分混合后接种到兔子体内。通过用相反类型的本周氏蛋白吸收获得针对κ链和λ链的单特异性抗血清。