Moulton B C, Koenig B B
Endocrinology. 1981 Feb;108(2):484-8. doi: 10.1210/endo-108-2-484.
Nuclear estrogen receptor levels were measured in fractions of dispersed deciduoma cells separated according to size by velocity sedimentation at unit gravity. Previous studies demonstrated that velocity sedimentation separated larger fully differentiated polyploid deciduoma cells from smaller diploid cells. Since levels of estrogen receptor measured in homogenates of deciduoma tissue decreased during the growth and differentiation of the deciduoma, lower levels of estrogen receptor per cell were anticipated in polyploid deciduoma cells. Although levels of estrogen receptor decreased slightly in all fractions of deciduoma cells from days 7-8 of pseudopregnancy, the larger polyploid cells had higher levels of estrogen receptor than smaller cells on both days. These results indicate that differentiation of endometrial stromal cells during decidualization involves a decrease in receptor levels in all deciduomal cell types rather than a specific decrease in the receptor levels of only fully differentiated deciduoma cells. (Endocrinology 108: 484, 1981)
采用单位重力下的速度沉降法，根据大小对分散的蜕膜瘤细胞进行分级分离，并测定各级分中核雌激素受体的水平。先前的研究表明，速度沉降法可将较大的、完全分化的多倍体蜕膜瘤细胞与较小的二倍体细胞分离开来。由于在蜕膜瘤组织匀浆中测得的雌激素受体水平在蜕膜瘤的生长和分化过程中会降低，因此预计多倍体蜕膜瘤细胞中每个细胞的雌激素受体水平会更低。尽管在假孕第7 - 8天，蜕膜瘤细胞的所有级分中雌激素受体水平均略有下降，但在这两天，较大的多倍体细胞的雌激素受体水平均高于较小的细胞。这些结果表明，蜕膜化过程中子宫内膜基质细胞的分化涉及所有蜕膜瘤细胞类型中受体水平的降低，而不仅仅是完全分化的蜕膜瘤细胞中受体水平的特异性降低。（《内分泌学》108: 484, 1981）
