Talley D J, Tobert J A, Armstrong E G, Villee C A
Endocrinology. 1977 Nov;101(5):1538-44. doi: 10.1210/endo-101-5-1538.
The presence of a cytoplasmic estradiol receptor with an affinity constant of 10(10)M-1 has been demonstrated in the differentiated fibroblasts comprising the deciduomata in pseudopregnant rats. The receptor had a sedimentation coefficient of 6S in sucrose density gradients containing a physiological concentration of salt, and estradiol binding was completely abolished by a hundred-fold excess of unlabeled estradiol. During the early stages of decidualization (days 2 and 3), the decidualized uterine horn or isolated deciduomal tissue contained a concentration of receptor comparable to that in non-gravid uteri from rats ovariectomized at estrus. By day 5 of decidualization, the concentration of estradiol binding in deciduomal tissue decreased to about one-half the concentration measurable at days 2 and 3 of decidualization despite continued tissue growth until day 7. By day 7 of decidualization, estradiol binding had decreased to about 20% of the concentration on days 2 and 3 of decidualization. Cytosol from untreated uterine horns of rats bearing deciduomata bound the same amount of estradiol through day 7 as that in uteri of rats ovariectomized at estrus. These observations are discussed in terms of steroid hormone involved in deciduomal growth and regression.
在假孕大鼠构成蜕膜瘤的分化成纤维细胞中，已证实存在一种亲和力常数为10(10)M-1的细胞质雌二醇受体。在含有生理浓度盐的蔗糖密度梯度中，该受体的沉降系数为6S，并且过量一百倍的未标记雌二醇可完全消除雌二醇结合。在蜕膜化早期（第2天和第3天），蜕膜化的子宫角或分离的蜕膜组织中受体浓度与动情期切除卵巢的大鼠非妊娠子宫中的受体浓度相当。到蜕膜化第5天，尽管组织持续生长直至第7天，但蜕膜组织中雌二醇结合浓度降至蜕膜化第2天和第3天可测量浓度的约二分之一。到蜕膜化第7天，雌二醇结合已降至蜕膜化第2天和第3天浓度的约20%。携带蜕膜瘤的大鼠未经处理的子宫角的胞质溶胶在第7天前结合的雌二醇量与动情期切除卵巢的大鼠子宫中的相同。将根据参与蜕膜生长和退化的类固醇激素来讨论这些观察结果。
