Golecki J R, Drews G
Eur J Cell Biol. 1980 Oct;22(2):654-60.
The obligatory halophilic, phototrophic bacterium, strain WS 68, grows under anaerobic conditions in the light in a mineral medium with acetate as carbon source and 6 to 20% sodium chloride, or in the same medium semiaerobically in the dark. The cells are vibrio- or spiral-shaped and show a bipolar polytrichous flagellation. The absorption spectrum of membranes, isolated from phototrophically grown cells, shows absorption maxima at 375, 490, 520, 555, 590, 800, 840-845 and 870-875 nm indicating the presence of bacteriochlorophyll a and carotenoids as pigments of the photosynthetic apparatus. The cell surface is covered with a layer of regular arranged particles (center to center distance approximately 10.5 nm). A murein (peptidoglycan) sacculus has not been detected in ultrathin sections, freeze fracture preparations and cells extracted with hot dodecyl sulfate. The intracytoplasmic membranes lie parallel to the cell envelope. The polypeptide patterns of isolated membranes and of the envelope fraction have been demonstrated. The results are discussed in the light of taxonomy and cell organization.
专性嗜盐光合细菌WS 68菌株，在以乙酸盐作为碳源且含有6%至20%氯化钠的矿物培养基中，于光照厌氧条件下生长，或者在相同培养基中黑暗半需氧条件下生长。细胞呈弧状或螺旋状，具双极多毛鞭毛。从光合生长细胞中分离出的膜的吸收光谱显示，在375、490、520、555、590、800、840 - 845和870 - 875纳米处有吸收最大值，表明存在细菌叶绿素a和类胡萝卜素作为光合装置的色素。细胞表面覆盖有一层规则排列的颗粒（中心距约10.5纳米）。在超薄切片、冷冻断裂标本以及用热十二烷基硫酸盐提取的细胞中均未检测到胞壁质（肽聚糖）囊。胞内膜与细胞膜平行。已展示了分离膜和包膜组分的多肽图谱。根据分类学和细胞组织对结果进行了讨论。
