Golecki J, Drews G, Bühler R
Cytobiologie. 1979 Feb;18(3):381-9.
By freeze-fracture electron microscopy, particles have been observed on the protoplasmic leaflet (PF face) of cytoplasmic and intracytoplasmic membranes of the photosynthetic bacterium Rhodopseudomonas capsulata. The particles are present under all culture conditions of chemotrophically and phototrophically grown cells. However, the number of particles per microM2 increased significantly when the formation of the photosynthetic apparatus in the membrane is induced. Intracytoplasmic membranes, where the bulk of photosynthetic activity is localized, always have a higher density of particles than cytoplasmic membranes. Under all conditions particles with a diameter of 9.5 nm dominate. The frequency of particles with diameters greater or smaller than 9.5 nm changed with culture conditions. A comparison of biochemical and electron microscopic data have lead us to the conclusion that the particles, formed under conditions which allow the synthesis of the photosynthetic apparatus, are composed of photochemical reaction centers and antenna light-harvesting bacteriochlorophyll I (B 875)-protein complexes. The total molecular weight of these particles is calculated to be 500,000.
通过冷冻断裂电子显微镜观察发现，在光合细菌荚膜红假单胞菌的细胞质膜和胞内膜的原生质小叶（PF面）上存在颗粒。这些颗粒在化能营养生长和光营养生长细胞的所有培养条件下均有出现。然而，当诱导膜中光合装置形成时，每平方微米的颗粒数量显著增加。光合活性主要集中的胞内膜，其颗粒密度总是高于细胞质膜。在所有条件下，直径为9.5纳米的颗粒占主导。直径大于或小于9.5纳米的颗粒频率随培养条件而变化。生化数据和电子显微镜数据的比较使我们得出结论，在允许光合装置合成的条件下形成的颗粒由光化学反应中心和天线光捕获细菌叶绿素I（B 875）-蛋白质复合物组成。这些颗粒的总分子量计算为500,000。
