Cinti D L
Res Commun Chem Pathol Pharmacol. 1975 Oct;12(2):339-54.
Addition of the cell soluble supernatant fraction to an assay medium containing NADPH generating system, mixed function oxidase substrate and microsomes, resulted in a stimulation of drug metabolism ranging from 12-75%. This stimulation was observed only when the supply of DADPH generating system (isocitric dehydrogenase or glucose 6 phosphate dehydrogenase) was insufficient, leading to a NADPH oxidation rate which was greater than the rate of reduction of NADP+ during the oxidation of a drug. Hence, under our assay conditions, the soluble supernate (SS) is only providing sufficient NADPH generator, and possibly relieving inhibition by the generated NADP+. Finally, microsomal lipid peroxidation measurements under these same conditions indicate negligible to no peroxidation activity in the absence of SS.
将细胞可溶性上清液组分添加到含有NADPH生成系统、混合功能氧化酶底物和微粒体的测定培养基中，导致药物代谢刺激增加12% - 75%。仅当DADPH生成系统（异柠檬酸脱氢酶或葡萄糖6磷酸脱氢酶）供应不足时才观察到这种刺激，这导致NADPH氧化速率大于药物氧化过程中NADP +的还原速率。因此，在我们的测定条件下，可溶性上清液（SS）仅提供足够的NADPH生成剂，并可能减轻所生成的NADP +的抑制作用。最后，在相同条件下进行的微粒体脂质过氧化测量表明，在没有SS的情况下，过氧化活性可忽略不计或没有。
