Whiting M J, Down R H, Watts J M
Gastroenterology. 1981 Mar;80(3):533-8.
The reproducibility of measurement of the cholesterol saturation index of duodenal bile has been investigated in 5 healthy male and 10 healthy female volunteers. Two possible causes for variation in the saturation index were examined. First, the precision of the assays for cholesterol, bile acids, and phospholipids was such that after replicate analysis of eight individual bile samples, analytic variation produced cholesterol saturation index values which covered a mean range of 0.09 +/- 0.01 (SEM). Secondly, biologic variation was observed in all volunteers when eight duodenal bile samples were collected on different days over a 1-month period. Biologic plus analytic variation accounted for large fluctuations in the biliary cholesterol saturation index, covering a mean range of 0.32 +/- 0.05 (SEM). The magnitude of both types of variation was the same for men and women and was not related to physiologic changes in sex hormone levels during the menstrual cycle. After allowing for analytic variation, the cholesterol saturation index of duodenal bile accurately reflected that of gallbladder bile when collected at the same time from gallstone patients at operation.
在5名健康男性和10名健康女性志愿者中，对十二指肠胆汁胆固醇饱和指数测量的可重复性进行了研究。研究了饱和指数变化的两个可能原因。首先，胆固醇、胆汁酸和磷脂检测的精密度使得对8份个体胆汁样本进行重复分析后，分析变异产生的胆固醇饱和指数值覆盖的平均范围为0.09±0.01（标准误）。其次，在1个月的时间内，在不同日期收集8份十二指肠胆汁样本时，观察到所有志愿者都存在生物学变异。生物学变异加上分析变异导致胆汁胆固醇饱和指数出现较大波动，覆盖的平均范围为0.32±0.05（标准误）。两种变异的幅度在男性和女性中相同，且与月经周期中性激素水平的生理变化无关。在考虑分析变异后，十二指肠胆汁的胆固醇饱和指数在手术时从胆结石患者同时采集胆囊胆汁时能准确反映胆囊胆汁的胆固醇饱和指数。
