Freedman P M, Ault B, Zeiss C R, Treuhaft M W, Roberts R C, Emanuel D A, Baldauf M C, Marx J J
J Allergy Clin Immunol. 1981 Jan;67(1):51-8. doi: 10.1016/0091-6749(81)90045-2.
Intradermal skin tests with a culture filtrate antigen of Micropolyspora faeni grown on a synthetic medium were performed on patients with farmers' lung disease (FLD) and well farmers with and without antibodies to a panel of FLD antigens. Seventy-five percent of the FLD patients, 79% of the well farmers with M. faeni antibody, and 5% of well farmers without M. faeni antibody had a 2+ or greater intradermal immediate skin-test reaction. Prausnitz-Küstner (P-K) reactions were positive using serum of M. faeni immediate skin test-positive FLD patients. IgG-rich fractions from a staphylococcal protein A-Sepharose column of such serum contained the sensitizing factor whereas IgG-depleted fractions did not. M. faeni-specific IgE could not be detected in serum by a polystyrene radioimmunoassay. Positive late-onset (6-hr) skin tests occurred only in FLD patients and farmers with precipitating antibody. Biopsy specimens of the 6-hr reactions revealed a generalized dermal and perivascular polymorphonuclear infiltrate with deposits of immunoglobulin and complement about blood vessels. The skin-sensitizing factor noted in FLD patients and well farmers with antibody is not disease specific. This factor appears to be associated with the IgG-rich fraction of serum, and its role in the pathogenesis of FLD is unclear.
用在合成培养基上培养的嗜热放线菌的培养滤液抗原，对农民肺疾病（FLD）患者以及有和没有一组FLD抗原抗体的健康农民进行皮内皮肤试验。75%的FLD患者、79%有嗜热放线菌抗体的健康农民以及5%没有嗜热放线菌抗体的健康农民皮内即刻皮肤试验反应为2+或更强。使用嗜热放线菌即刻皮肤试验阳性的FLD患者的血清，普劳斯尼茨-屈斯特纳（P-K）反应呈阳性。来自这种血清的葡萄球菌蛋白A-琼脂糖柱的富含IgG的组分含有致敏因子，而去除IgG的组分则没有。通过聚苯聚苯聚苯烯丙基胺基聚苯乙烯放射免疫测定法在血清中未检测到嗜热放线菌特异性IgE。迟发性（6小时）皮肤试验阳性仅发生在有沉淀抗体的FLD患者和农民中。6小时反应的活检标本显示广泛的真皮和血管周围多形核浸润，血管周围有免疫球蛋白和补体沉积。在FLD患者和有抗体的健康农民中发现的皮肤致敏因子并非疾病特异性的。该因子似乎与血清中富含IgG的组分有关，其在FLD发病机制中的作用尚不清楚。
