在神经节苷脂分离过程中使用Sep-Pak C18柱。
The use of Sep-Pak C18 cartridges during the isolation of gangliosides.
作者信息
Williams M A, McCluer R H
出版信息
J Neurochem. 1980 Jul;35(1):266-9. doi: 10.1111/j.1471-4159.1980.tb12515.x.
Abstract
The use of Sep-Pak C18 reverse-phase cartridges to adsorb gangliosides from aqueous solutions was studied. When upper phases formed from chloroform-methanol tissue extracts or aqueous salt solutions containing gangliosides are rapidly passed through the cartridges, the lipids are retained and the non-lipid components can be washed through. Gangliosides and other retained lipids can subsequently be eluted with methanol or chloroform-methanol
摘要
研究了使用Sep-Pak C18反相柱从水溶液中吸附神经节苷脂的情况。当由氯仿-甲醇组织提取物或含有神经节苷脂的盐水溶液形成的上层相快速通过柱子时，脂质被保留，非脂质成分可以被冲洗掉。随后，神经节苷脂和其他保留的脂质可用甲醇或氯仿-甲醇洗脱。
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