通过自动多次展开改进神经节苷脂的薄层色谱分离

Improved thin-layer chromatographic separation of gangliosides by automated multiple development.

作者信息

Müthing J

机构信息

Institut für Zellkulturtechnik, Universität Bielefeld, Germany.

出版信息

J Chromatogr B Biomed Appl. 1994 Jul 1;657(1):75-81. doi: 10.1016/0378-4347(94)80072-3.

DOI:10.1016/0378-4347(94)80072-3

PMID:7952086

Abstract

Automated multiple development chromatography has been utilized to enhance separation of gangliosides on silica-gel precoated high-performance TLC plates. Three-fold chromatography of a complex mixture of neolacto-series monosialogangliosides in the solvent chloroform-methanol-water (120:85:14, v/v, 2 mM CaCl2) resulted in a ca. three-fold increase in separation distance of e.g. alpha 2-3 and alpha 2-6 sialylated ganglioside isomers compared to conventional single chromatography in the standard solvent chloroform-methanol-water (120:85:20, v/v, 2 mM CaCl2). An extremely heterogenous murine disialoganglioside mixture was developed three times in chloroform-methanol-water (120:85:16, v/v, 2 mM CaCl2) leading to a more than two-fold increase in separation distance. Chloroform-methanol-water (120:85:22, v/v, 2 mM CaCl2) was the solvent of choice for multiple chromatography of ganglio-series polysialogangliosides from embryonic chicken brain.

摘要

自动多次展开色谱法已被用于增强神经节苷脂在硅胶预涂高性能薄层色谱板上的分离效果。在氯仿 - 甲醇 - 水（120:85:14，v/v，2 mM氯化钙）溶剂中对新乳糖系列单唾液酸神经节苷脂的复杂混合物进行三次展开色谱，与在标准溶剂氯仿 - 甲醇 - 水（120:85:20，v/v，2 mM氯化钙）中进行的传统单次色谱相比，例如α2 - 3和α2 - 6唾液酸化神经节苷脂异构体的分离距离增加了约三倍。在氯仿 - 甲醇 - 水（120:85:16，v/v，2 mM氯化钙）中对一种极其异质的小鼠双唾液酸神经节苷脂混合物进行三次展开，导致分离距离增加了两倍多。氯仿 - 甲醇 - 水（120:85:22，v/v，2 mM氯化钙）是用于对来自胚胎鸡脑的神经节系列多唾液酸神经节苷脂进行多次色谱的首选溶剂。