Goodrum J F, Tanaka R
Neurochem Res. 1978 Oct;3(5):599-617. doi: 10.1007/BF00963762.
Highly enriched fractions of synaptic junctional complexes, synaptic vesicles, and coated vesicles were isolated from rat forebrains and compared, along with synaptosomal plasma membrane and its nonjunctional components, by discontinuous sodium dodecylsulfate-polyacrylamide gel electrophoresis. When stained for proteins with Coomassie blue, the gels all contained the same protein bands, only in different relative amounts. Mixing experiments revealed no additional bands. However, gel patterns of each fraction were not only quantitatively but also qualitatively different when stained for carbohydrate. These observations suggested that some of the protein bands may vary in their degree of glycosylation among the various synaptic fractions. Within the limits of resolution of the methods used here, these results are consistent with the morphological process of synaptic vesicle exocytosis and recycling but suggest the possibility of a reversible modification of certain membrane glycoproteins as they pass through the various membrane compartments.
从大鼠前脑中分离出高度富集的突触连接复合体、突触小泡和被膜小泡组分，并与突触体细胞膜及其非连接组分一起，通过不连续十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行比较。用考马斯亮蓝对蛋白质进行染色时，所有凝胶都含有相同的蛋白条带，只是相对含量不同。混合实验未发现额外的条带。然而，当对碳水化合物进行染色时，每个组分的凝胶图谱不仅在数量上而且在质量上都有所不同。这些观察结果表明，某些蛋白条带在不同的突触组分中糖基化程度可能不同。在本文所用方法的分辨率范围内，这些结果与突触小泡胞吐和再循环的形态学过程一致，但表明某些膜糖蛋白在穿过不同膜区室时可能发生可逆修饰。
