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神经细胞培养中的超氧化物歧化酶活性

Superoxide dismutase activity in nerve cell culture.

作者信息

Fried R, Ciesielski-Treska J, Ledig M, Mandel P

出版信息

Neurochem Res. 1978 Oct;3(5):633-9. doi: 10.1007/BF00963764.

DOI:10.1007/BF00963764
PMID:745667
Abstract

Superoxide dismutase (EC 1.15.1.1) activity was assayed in preparations obtained from several clonal lines of nerve cell culture, by enzymatic and nonenzymatic assays. The enzyme was found in dialyzed homogenates of washed cells and in partially purified fractions. The enzyme was also found in cells which had been grown in media containing 5-bromodeoxyuridine, where cell differentiation was observed.

摘要

通过酶促和非酶促测定法,对从几种神经细胞培养克隆系获得的制剂中的超氧化物歧化酶(EC 1.15.1.1)活性进行了测定。在洗涤过的细胞的透析匀浆和部分纯化级分中发现了该酶。在含有5-溴脱氧尿苷的培养基中生长且观察到细胞分化的细胞中也发现了该酶。

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引用本文的文献

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Neurochem Res. 1980 Nov;5(11):1155-62. doi: 10.1007/BF00964895.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Superoxide dismutases.超氧化物歧化酶
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Superoxide dismutase of mammalian nervous system.哺乳动物神经系统的超氧化物歧化酶
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Enzymic modifications in a cultivated neuroblastoma clone after bromodeoxyuridine treatment.经溴脱氧尿苷处理后培养的神经母细胞瘤克隆中的酶促修饰。
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