Rollhäuser-ter Horst J
Anat Embryol (Berl). 1980;160(2):203-11. doi: 10.1007/BF00301861.
Early Axolotl gastrula ectoderm was grafted into early Triturus neural stages in place of excised neural folds at the gill and anterior trunk level. Macroscopically the young graft behaves like normal neural fold material: it follows the closing host neural plate to the dorsal midline, folds into the host's interior and, especially in the gill region, moves ventrad beneath the host's epidermis. These movements cannot be interpreted as active migration. They are the result of passive displacements by morphogenetic forces inside the embryo. Histologically the graft differentiates into neural and neural crest tissue, the quantitative relation depending on the host's region. At the gill level the graft forms mesenchyme and other neural crest elements and hardly any neural structures. In the trunk about one half of the graft forms a secondary, surplus CNS. Problems of induction, differences between gill and trunk region and between graft and normal fold behaviour are discussed. Limbs develop normally. The dorsal layer of the blastema is furnished by graft cells. Host and graft tissue can stay separate or form a combined blastema.
将早期蝾螈原肠胚外胚层移植到处于神经形成早期阶段的美西螈的鳃和躯干前部水平，以替代切除的神经褶。从宏观上看，年轻的移植组织表现得如同正常的神经褶组织：它随着宿主神经板闭合至背中线，折叠进入宿主内部，尤其是在鳃区域，在宿主表皮下方腹侧移动。这些运动不能被解释为主动迁移。它们是胚胎内部形态发生力导致的被动位移的结果。从组织学上看，移植组织分化为神经组织和神经嵴组织，其数量关系取决于宿主区域。在鳃水平，移植组织形成间充质和其他神经嵴成分，几乎不形成任何神经结构。在躯干中，大约一半的移植组织形成一个次生的、多余的中枢神经系统。文中讨论了诱导问题、鳃和躯干区域之间以及移植组织和正常褶行为之间的差异。肢体正常发育。芽基的背层由移植细胞提供。宿主组织和移植组织可以保持分离或形成联合芽基。
