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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Rollhäuser-ter Horst J

Anat Embryol (Berl). 1977 Dec 2;151(3):309-16. doi: 10.1007/BF00318932.

Embryonic tissues (ectoderm, neural plate) of Ambystoma mexicanum and Tritus were killed with hot water and implanted into ectoderm sandwiches. They induced the ectoderm to form neural tissue, lentoids and unspecialized epidermis. Neural tissue always showed retina character. Egg pigment was eliminated and gathered at the outer retinal surface or in the centre of rosettes. Neural crest cells like mesenchyme or melanophores were completely lacking, retinal pigment did not develop. 2. The thus induced living retina tissue was reimplanted into fresh ectoderm after 2 days. It continued histogenetic and morphogenetic differentiation and formed ocular vesicles with numerous eye cups. It induced the enveloping ectoderm to again form retina, lentoids and unspecialized epidermis without neural crest derivatives or RPE. 3. This inductive process can be reproduced several times.

用热水杀死美西螈和蝾螈的胚胎组织（外胚层、神经板），并将其植入外胚层三明治中。它们诱导外胚层形成神经组织、晶状体和未分化的表皮。神经组织总是呈现视网膜特征。卵色素被清除并聚集在视网膜外表面或玫瑰花结中心。完全缺乏像间充质或黑素细胞那样的神经嵴细胞，视网膜色素未发育。2. 这样诱导形成的活视网膜组织在2天后被重新植入新鲜的外胚层中。它继续进行组织发生和形态发生分化，并形成带有许多眼杯的眼泡。它诱导包绕的外胚层再次形成视网膜、晶状体和未分化的表皮，而没有神经嵴衍生物或视网膜色素上皮。3. 这个诱导过程可以重复多次。

Rollhäuser-ter Horst J

Anat Embryol (Berl). 1977 Dec 2;151(3):309-16. doi: 10.1007/BF00318932.

Embryonic tissues (ectoderm, neural plate) of Ambystoma mexicanum and Tritus were killed with hot water and implanted into ectoderm sandwiches. They induced the ectoderm to form neural tissue, lentoids and unspecialized epidermis. Neural tissue always showed retina character. Egg pigment was eliminated and gathered at the outer retinal surface or in the centre of rosettes. Neural crest cells like mesenchyme or melanophores were completely lacking, retinal pigment did not develop. 2. The thus induced living retina tissue was reimplanted into fresh ectoderm after 2 days. It continued histogenetic and morphogenetic differentiation and formed ocular vesicles with numerous eye cups. It induced the enveloping ectoderm to again form retina, lentoids and unspecialized epidermis without neural crest derivatives or RPE. 3. This inductive process can be reproduced several times.

用热水杀死美西螈和蝾螈的胚胎组织（外胚层、神经板），并将其植入外胚层三明治中。它们诱导外胚层形成神经组织、晶状体和未分化的表皮。神经组织总是呈现视网膜特征。卵色素被清除并聚集在视网膜外表面或玫瑰花结中心。完全缺乏像间充质或黑素细胞那样的神经嵴细胞，视网膜色素未发育。2. 这样诱导形成的活视网膜组织在2天后被重新植入新鲜的外胚层中。它继续进行组织发生和形态发生分化，并形成带有许多眼杯的眼泡。它诱导包绕的外胚层再次形成视网膜、晶状体和未分化的表皮，而没有神经嵴衍生物或视网膜色素上皮。3. 这个诱导过程可以重复多次。