Sharawy M M, Liebelt A G, Dirksen T R, Penney D P
Anat Rec. 1980 Sep;198(1):125-33. doi: 10.1002/ar.1091980110.
Postcastrational adrenocortical carcinomas in the CE/Ki inbred strains of mice and the adrenals of noncastrated CE/Ki mice were studied using light and electron microscopic techniques. Most of the tumors appeared as large nodules of cells separated by septae comprised of collagen and blood sinusoids. The majority of tumor cells (Type 1) showed few or no lipid droplets (sudanophobic), polymorphic hyperchromatic nuclei, lack of SER, abundant RER and free ribosomes, prominent Golgi complexes, and few mitochondria with scant internal membranes. Clusters of Type 1 cells were surrounded by a basal lamina. In contrast, Type 2 cells revealed abundant and dilated tubules of SER, large number of lipid droplets and mitochondria with tubulovesicular cristae. These results suggest that Type 2 cells were probably active in steroid hormone synthesis and secretion while Type 1 cells were highly anaplastic and apparently non-steroid-secreting cells.
利用光镜和电镜技术，对CE/Ki近交系小鼠的去势后肾上腺皮质癌以及未去势CE/Ki小鼠的肾上腺进行了研究。大多数肿瘤表现为大的细胞结节，被由胶原和血窦组成的间隔分隔。大多数肿瘤细胞（1型）几乎没有或没有脂滴（嗜苏丹阴性），核多形性、深染，缺乏滑面内质网，粗面内质网和游离核糖体丰富，高尔基体复合体突出，线粒体数量少且内膜稀少。1型细胞簇被基膜包围。相比之下，2型细胞显示滑面内质网丰富且扩张，有大量脂滴和具有管状嵴的线粒体。这些结果表明，2型细胞可能在类固醇激素合成和分泌中活跃，而1型细胞是高度间变的且显然是非类固醇分泌细胞。
