Suzuki N, Williams M, Hunter N M, Withers H R
Br J Cancer. 1980 Nov;42(5):765-71. doi: 10.1038/bjc.1980.310.
Freshly isolated cones of high cloning efficiency from a mouse fibrosarcoma were examined for DNA content, cell size, protein content, and malignant characteristics such as artificial lung-colony-forming ability, s.c. tumour take, host survival, and spontaneous metastatic ability. These malignant characteristics and other cell properties were heterogeneous among these clones; the malignant characteristics could vary and were not "all or none" in their nature. The higher the DNA content or the larger the cell volume, the higher the malignancy in terms of artificial lung-colony forming, efficiency, s.c. tumour take, and host survival. Despite variability of each parameter, the ratio of DNA content to cell size or protein content remained constant through these variations: the increased DNA paralleled increased protein and increased cell volume. The increased DNA was correlated with the more malignant characteristics of local growth and lung-colony-forming efficiency. Spontaneous metastasis to the lung was totally different from the local growth abilities; the small-cell clone produced more metastases. The graded nature of malignant properties and the differentiation between local growth and metastatic potential among the daughter clones indicate that malignancy reflects a complex moiety of cell properties.
从一只小鼠纤维肉瘤中分离出具有高克隆效率的新鲜锥体细胞，检测其DNA含量、细胞大小、蛋白质含量以及恶性特征，如人工肺集落形成能力、皮下肿瘤接种成功率、宿主存活率和自发转移能力。这些恶性特征和其他细胞特性在这些克隆之间是异质的；恶性特征可能会有所不同，本质上并非“全有或全无”。就人工肺集落形成、效率、皮下肿瘤接种成功率和宿主存活率而言，DNA含量越高或细胞体积越大，恶性程度越高。尽管每个参数存在变异性，但DNA含量与细胞大小或蛋白质含量的比值在这些变化中保持恒定：DNA增加与蛋白质增加和细胞体积增加并行。DNA增加与局部生长和肺集落形成效率的更恶性特征相关。肺的自发转移与局部生长能力完全不同；小细胞克隆产生更多转移灶。子代克隆中恶性特性的分级性质以及局部生长和转移潜能之间的差异表明，恶性反映了细胞特性的复杂部分。
