Böhmer R M
Cell Tissue Kinet. 1980 Sep;13(5):497-503. doi: 10.1111/j.1365-2184.1980.tb00490.x.
Colcemid was added to the growth medium of L-cells in monolayer culture. The proliferating cells continued their progression through the cycle up to metaphase, where they were arrested. At different times after Colcemid addition the cells were trypsinized, suspended immediately in a solution of the DNA-specific fluorescent dye Hoechst 33258 and analysed with a flow cytometer. The histograms were evaluated to give the fraction of cells in the 2c peak as a function of time after Colcemid addition. The flux into the 2c compartment being interrupted, the peak content decreased until all proliferating (G1) cells had entered S-phase. With increasing cell density or with increasing time after serum deprivation an increasing fraction of cells remained in the 2c peak at times greater than the normal G1 duration. The possibility of applying this method to the determination of non-proliferating cells in a population is discussed.
秋水仙酰胺被添加到单层培养的L细胞的生长培养基中。增殖细胞继续其细胞周期进程直至中期，此时它们被阻滞。在添加秋水仙酰胺后的不同时间，将细胞用胰蛋白酶消化，立即悬浮于DNA特异性荧光染料Hoechst 33258溶液中，并用流式细胞仪进行分析。对直方图进行评估，以给出在添加秋水仙酰胺后作为时间函数的2c峰中细胞的比例。进入2c区室的通量被中断，峰含量下降，直到所有增殖（G1）细胞进入S期。随着细胞密度增加或血清剥夺后时间延长，在大于正常G1持续时间的时间点，留在2c峰中的细胞比例增加。讨论了将该方法应用于测定群体中非增殖细胞的可能性。
