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New approach to brain tumour chemoradiotherapy with cellular synchronization by colcemid.

作者信息

Shitara N, Kohno T, Takakura K

出版信息

Acta Neurochir (Wien). 1976;35(1-3):123-33. doi: 10.1007/BF01405940.

DOI:10.1007/BF01405940
PMID:961496
Abstract

Cellular synchronization using Colcemid as pretreatment for combined chemoradiotherapy was investigated. C6 rat brain tumour was cultured in RPMI medium containing 10(-5)-10(-7) Mol. of Colcemid for 24 hours. The basic cell kinetics were analysed with a Pulse Cytophotometer, which facilitated the analysis of tumour cell cycle phase distribution according to the DNA content. The effect of Colcemid depended on the concentration, and the minimal concentration showing continuous blocking during 48 hours after removal of the drug was 10(-6) Mol. G1 fraction of 2 C DNA content was reduced from 74% to 36%. G2-M phase of 4 C DNA content increased from 9% to 28%. S phase cells increased from 17% to 31%. Polyploid cells in the Tetraploid cell cycle could be recognized. The remaining 36% of cells within the GO + G1 peak of 2 C DNA content were considered to be diploid GO cells.

摘要

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New approach to brain tumour chemoradiotherapy with cellular synchronization by colcemid.
Acta Neurochir (Wien). 1976;35(1-3):123-33. doi: 10.1007/BF01405940.
2
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Cytometry. 1998 Apr 1;31(4):295-9. doi: 10.1002/(sici)1097-0320(19980401)31:4<295::aid-cyto9>3.0.co;2-i.
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J Natl Cancer Inst. 1979 Jul;63(1):67-70.
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本文引用的文献

1
THE MECHANISM OF COLCHICINE INHIBITION OF MITOSIS. I. KINETICS OF INHIBITION AND THE BINDING OF H3-COLCHICINE.秋水仙碱抑制有丝分裂的机制。I. 抑制动力学及H3-秋水仙碱的结合
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LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION.哺乳动物细胞的生命周期分析。I. 一种在生命周期内定位代谢事件的方法及其在秋水仙酰胺作用和X射线亚致死剂量照射中的应用。
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Variations in several responses of HeLa cells to x-irradiation during the division cycle.
在分裂周期中，海拉细胞对X射线照射的几种反应的变化。
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A colchicine derivative in therapy of chronic myelocytic leukemia.一种秋水仙碱衍生物在慢性粒细胞白血病治疗中的应用。
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Arch Klin Exp Dermatol. 1971;239(4):377-89.
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Implications of biochemical, cytokinetic, pharmacologic, and toxicologic relationships in the design of optimal therapeutic schedules.生化、细胞动力学、药理学及毒理学关系在优化治疗方案设计中的意义。
Cancer Chemother Rep. 1970 Dec;54(6):431-50.
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[Synchronization of the mammalian cell division cycle in vitro using fractionated x-ray irradiation].[利用分次X射线照射在体外同步哺乳动物细胞分裂周期]
Strahlentherapie. 1970 Dec;140(6):706-10.
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Synchronisation in vivo: kinetics of a malignant cell system following temporary inhibition of DNA synthesis with hydroxyurea.
Exp Cell Res. 1970 May;60(2):269-76. doi: 10.1016/0014-4827(70)90514-8.