Archer M C, Eng V W
Chem Biol Interact. 1981 Jan;33(2-3):207-14. doi: 10.1016/0009-2797(81)90041-7.
Reaction of nitrosodiethylamine (NDEA) in a non-enzymatic, chemical activation system consisting of ascorbic acid, ferrous ions, EDTA and molecular oxygen yielded an alkylating intermediate that was detected by its reaction with 4-(p-nitrobenzyl)pyridine (PNBP) to yield a colored product. Reaction conditions for the chemical activation-detection system were optimized, allowing detection of approx. 10 mumol NDEA. The method detected a variety of acyclic and cyclic nitrosamines and other compounds that become alkylating agents only following oxidative metabolism.
在由抗坏血酸、亚铁离子、乙二胺四乙酸和分子氧组成的非酶促化学活化体系中，亚硝基二乙胺（NDEA）反应生成一种烷基化中间体，该中间体通过与4-（对硝基苄基）吡啶（PNBP）反应产生有色产物来进行检测。对化学活化检测体系的反应条件进行了优化，能够检测到约10微摩尔的NDEA。该方法检测到了多种无环和环状亚硝胺以及其他仅在氧化代谢后才成为烷基化剂的化合物。
