苯并[a]芘对哺乳动物细胞（CHO/HGPRT系统）细胞毒性和致突变性的定量分析。

Quantitative analysis of cytotoxicity and mutagenicity of benzo[a]pyrene in mammalian cells (CHO/HGPRT system).

作者信息

Machanoff R, O'Neill J P, Hsie A W

出版信息

Chem Biol Interact. 1981 Feb;34(1):1-10. doi: 10.1016/0009-2797(81)90084-3.

Abstract

Chinese hamster ovary (CHO) cells in cell culture were utilized to study the cytotoxic and mutagenic activity of benzo[a]pyrene (BP). A liver homogenate (S9) prepared from Arochlor 1254 induced male Sprague-Dawley rats was employed to mediate the biotransformation of the promutagen. The dependence of the induction of 6-thioguanine(TG)-resistant mutants on parameters such as BP concentration, amount of S9 protein, ionic composition of the S9 mix and treatment time was determined. Under optimal conditions with a 5-h treatment time, BP mutagenicity can be described as 185 mutants/10(6) cells per microM BP per mg/ml S9 protein.

摘要

利用细胞培养中的中国仓鼠卵巢（CHO）细胞来研究苯并[a]芘（BP）的细胞毒性和诱变活性。使用从经Arochlor 1254诱导的雄性Sprague-Dawley大鼠制备的肝匀浆（S9）来介导前诱变剂的生物转化。确定了6-硫鸟嘌呤（TG）抗性突变体的诱导对诸如BP浓度、S9蛋白量、S9混合物的离子组成和处理时间等参数的依赖性。在处理时间为5小时的最佳条件下，BP的诱变性可描述为每微摩尔BP每毫克/毫升S9蛋白产生185个突变体/10(6)个细胞。