Garrido A B, Freeman H J, Kim Y S
Dig Dis Sci. 1981 Feb;26(2):107-12. doi: 10.1007/BF01312225.
The intestinal digestive and absorptive function of the excluded jejunum was evaluated 6 and 12 weeks after jejunoileal bypass in rats. Brush border and cytosol aminopeptidase activities as well as mucosal sucrase activity were measured in self-emptying excluded jejunal segments following bypass surgery. In addition, following in vivo perfusion of equimolar amounts of amino acid (L-leucine) and dipeptide (L-leucyl-L-leucine) solutions through bypassed jejunal segments, the uptake of L-leucine was determined. Mucosal weight, protein, and DNA content were reduced in the bypass segment reflecting jejunal mucosal cell hypoplasia. Hydrolytic activities for all enzymes (including the subcellular fractions of the aminopeptidase activities) and absorption rates from both the free amino acid and peptide solutions were reduced in bypassed jejunal segments. When expressed on the basis of mucosal DNA content, however, no changes were observed. This study indicates that the functional alterations in the excluded jejunum after bypass are due to the reduced mucosal cell content of this segment.
在大鼠空肠回肠旁路术后6周和12周，评估被旷置空肠的肠道消化和吸收功能。在旁路手术后，对自行排空的被旷置空肠段进行刷状缘和胞质氨基肽酶活性以及黏膜蔗糖酶活性的测定。此外，通过被旁路的空肠段对等量的氨基酸（L-亮氨酸）和二肽（L-亮氨酰-L-亮氨酸）溶液进行体内灌注后，测定L-亮氨酸的摄取量。旁路段的黏膜重量、蛋白质和DNA含量降低，反映出空肠黏膜细胞发育不全。被旁路的空肠段中所有酶的水解活性（包括氨基肽酶活性的亚细胞部分）以及游离氨基酸和肽溶液的吸收率均降低。然而，以黏膜DNA含量为基础表示时，未观察到变化。本研究表明，旁路术后被旷置空肠的功能改变是由于该段黏膜细胞含量减少所致。
