Ramulu K S, Schibilla H, Dijkhuis P
Environ Health Perspect. 1981 Jan;37:43-51. doi: 10.1289/ehp.813743.
The self-incompatibility system of Oenothera organensis is used to analyze the frequency of S locus mutations induced by low doses (2.5-20 rad) of fast neutrons and x-rays. Th principles and methods of the screening system for detection of low dose effects have been presented. The results show that low doses induced significantly higher frequencies of seeds and seedlings than those produced spontaneously. The analyses of part of the progenies derived from the control and treated series suggested that they were due to revertible mutations. A modified in vitro method of culturing pollinated cut styles has been developed for rapid screening of compatible pollen tubes.
利用鄂报春（Oenothera organensis）的自交不亲和系统来分析低剂量（2.5 - 20拉德）快中子和X射线诱导的S位点突变频率。文中介绍了用于检测低剂量效应的筛选系统的原理和方法。结果表明，低剂量诱导产生的种子和幼苗频率显著高于自发产生的频率。对来自对照系列和处理系列的部分后代的分析表明，这些是由可回复突变引起的。已开发出一种改良的体外培养授粉花柱的方法，用于快速筛选亲和的花粉管。
