Hillier S, Charnetzky W T
J Bacteriol. 1981 Jan;145(1):452-8. doi: 10.1128/jb.145.1.452-458.1981.
Isocitrate lyase and malate synthase, the two unique enzymes of the glyoxylate cycle, were detected in crude extracts of Yersinia pestis, Y. pseudotuberculosis, and Y. enterocolitica. Y. pestis, unlike Escherichia coli and the other yersiniae tested, yielded two forms of isocitrate lyase during growth on acetate. These forms differed in electrophoretic mobility and temperature optima. One form (A) was present during growth on acetate, but was absent during growth on alternate carbon sources such as glucose. The second form (B) was not constitutive, but was found during growth on acetate, glucose, xylose, or other complex carbon sources. Itaconate, a succinate analog which inhibited both forms of isocitrate lyase in crude extracts, did not affect the growth of Y. pestis under conditions where little isocitrate lyase activity was detected. This inhibitor, however, retarded the growth of Y. pestis under conditions where acetate was provided as the primary carbon and energy source as well as under all conditions in which either form of isocitrate lyase was evident. This suggests that the B form may play an important role in the growth of this bacterium under conditions where a requirement for the classical anaplerotic sequence involving this enzyme is not apparent.
在鼠疫耶尔森菌、假结核耶尔森菌和小肠结肠炎耶尔森菌的粗提取物中检测到了异柠檬酸裂解酶和苹果酸合酶，这两种是乙醛酸循环特有的酶。与大肠杆菌和其他受试耶尔森菌不同，鼠疫耶尔森菌在以乙酸盐为碳源生长时产生了两种形式的异柠檬酸裂解酶。这两种形式在电泳迁移率和最适温度方面存在差异。一种形式（A）在以乙酸盐为碳源生长时存在，但在以葡萄糖等其他碳源生长时不存在。第二种形式（B）不是组成型的，而是在以乙酸盐、葡萄糖、木糖或其他复杂碳源生长时被发现。衣康酸是琥珀酸类似物，可抑制粗提取物中两种形式的异柠檬酸裂解酶，但在检测到极低异柠檬酸裂解酶活性的条件下，它对鼠疫耶尔森菌的生长没有影响。然而，在以乙酸盐作为主要碳源和能源的条件下以及在任何一种形式的异柠檬酸裂解酶明显存在的所有条件下，这种抑制剂都会阻碍鼠疫耶尔森菌的生长。这表明，在对涉及该酶的经典回补序列需求不明显的条件下，B形式可能在这种细菌的生长中发挥重要作用。
