种间杂交揭示的Podospora属物种核糖体成分的遗传学

Perrot M, Begueret J

J Bacteriol. 1981 Jan;145(1):82-7. doi: 10.1128/jb.145.1.82-87.1981.

The ribosomal proteins of several species within the genus Podospora have been analyzed by two-dimensional polyacrylamide gel electrophoresis. Differences in the migration of four proteins of the large subunit have been found between the two hybridizable species Podospora anserina and P. comata. Haploid offspring of this interspecific cross show all possible combinations of ribosomal proteins from both species. Therefore, the homologous proteins are functionally interchangeable. Moreover, the sizes of the different classes of offspring show that the genes encoding the four ribosomal proteins L2, L13, L16, and L20 are not clustered.

通过二维聚丙烯酰胺凝胶电泳分析了Podospora属内几个物种的核糖体蛋白。在两个可杂交的物种——嗜热栖热放线菌（Podospora anserina）和P. comata之间，发现了大亚基的四种蛋白质迁移存在差异。这种种间杂交的单倍体后代显示出来自两个物种的核糖体蛋白的所有可能组合。因此，同源蛋白在功能上是可互换的。此外，不同类别的后代大小表明，编码四种核糖体蛋白L2、L13、L16和L20的基因不是成簇的。