Adoutte-Panvier A, Davies J E, Gritz L R, Littlewood B S
Mol Gen Genet. 1980;179(2):273-82. doi: 10.1007/BF00425454.
The cytoplasmic ribosomal proteins (r-proteins) of seventeen yeast species of the genera Saccharomyces and Kluyveromyces were analyzed by one-dimensional gradient polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The electrophoretic patterns of cytoplasmic r-proteins from different species display extensive differences in both the 40S and the 60S subunit. Relatedness of species suggested by r-protein patterns correlates well with that based on DNA/DNA homology (Bicknell and Douglas 1970). Immunochemical cross-reactions and antibiotic susceptibility tests were also used to compare different species. Analyses of r-proteins from two different interspecific hybrids showed that their ribosomes were hybrid, containing r-proteins from both parents. These findings are discussed in relation to the evolution of yeast species and the regulation of expression of r-proteins in eucaryotes.
在十二烷基硫酸钠存在的情况下，通过一维梯度聚丙烯酰胺凝胶电泳分析了酿酒酵母属和克鲁维酵母属的17种酵母的细胞质核糖体蛋白（r蛋白）。不同物种细胞质r蛋白的电泳图谱在40S和60S亚基上均显示出广泛差异。r蛋白模式所表明的物种亲缘关系与基于DNA/DNA同源性的亲缘关系密切相关（Bicknell和Douglas，1970年）。免疫化学交叉反应和抗生素敏感性试验也用于比较不同物种。对两种不同种间杂种的r蛋白分析表明，它们的核糖体是杂种的，含有来自双亲的r蛋白。结合酵母物种的进化和真核生物中r蛋白表达的调控对这些发现进行了讨论。
