Immunological comparison of individual ribosomal proteins in six species of the genus Podospora.

The ribosomal proteins of six species belonging to the genus Podospora have been compared by electrophoretical and immunological methods. The important variability in the electrophoretical properties is similar to other lower eukaryotes but we could specify the extent of the variability of the r-proteins by the analysis of immunological relationships between individual proteins. Radioimmuno-detection of ribosomal proteins blotted on nitrocellulose sheets after separation on two-dimensional gels, was used to determine common antigenic sites on the r-proteins of the different species. These sites may be present on proteins that do or do not have identical electrophoretical mobilities. Furthermore, the proteins which co-migrate do not necessarily cross-react.