盘基网柄菌中的内源性细胞表面凝集素：定量、糖洗脱及二价免疫球蛋白激发
Endogenous cell surface lectin in Dictyostelium: quantitation, elution by sugar, and elicitation by divalent immunoglobulin.
作者信息
Springer W R, Haywood P L, Barondes S H
出版信息
J Cell Biol. 1980 Dec;87(3 Pt 1):682-90. doi: 10.1083/jcb.87.3.682.
Abstract
The amount of total endogenous cellular and cell surface lectin in aggregating Dictyostelium purpureum was determined by a number of immunochemical techniques. The results show that of the 5 x 10(6) molecules of the lectin (called purpurin) per aggregating cell only about 2% (1 x 10(5) molecules) is present on the cell surface. Cell surface purpurin can be specially eluted by lactose, which indicates that it is held to the surface by its carbohydrate-binding site. The eluted purpurin is replaced on the cell surface within 45 min. Estimates of cell surface purpurin made by binding of specific immunoglobulin to the cells at 4 degrees C indicate that a much larger amount, about 1 x 10(6) molecules, becomes associated with the cell surface in the presence of this divalent ligand. In contrast, univalent antibody fragments do not have this effect.
摘要
通过多种免疫化学技术测定了聚集的紫盘基网柄菌中内源性细胞和细胞表面凝集素的总量。结果表明，每个聚集细胞中有5×10⁶个凝集素分子（称为紫盘菌素），其中只有约2%（1×10⁵个分子）存在于细胞表面。细胞表面的紫盘菌素可以被乳糖特异性洗脱，这表明它通过其碳水化合物结合位点与表面结合。洗脱后的紫盘菌素在45分钟内会重新出现在细胞表面。在4℃下通过特异性免疫球蛋白与细胞结合对细胞表面紫盘菌素进行的估计表明，在这种二价配体存在的情况下，会有大量（约1×10⁶个分子）与细胞表面结合。相比之下，单价抗体片段没有这种作用。
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