细胞抗原在十二烷基硫酸钠-聚丙烯酰胺凝胶中的定位
Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.
作者信息
Adair W S, Jurivich D, Goodenough U W
出版信息
J Cell Biol. 1978 Oct;79(1):281-5. doi: 10.1083/jcb.79.1.281.
Abstract
A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.
摘要
本文描述了一种使用来自金黄色葡萄球菌的125I标记蛋白A在十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶中定位抗原-抗体复合物的方法。我们使用该方法探究了海胆和衣藻α-及β-微管蛋白之间的抗原交叉反应性；我们还展示了该方法如何检测针对细胞膜的抗血清中的次要抗体种类。
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THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
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