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细胞抗原在十二烷基硫酸钠-聚丙烯酰胺凝胶中的定位

Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.

作者信息

Adair W S, Jurivich D, Goodenough U W

出版信息

J Cell Biol. 1978 Oct;79(1):281-5. doi: 10.1083/jcb.79.1.281.

DOI:10.1083/jcb.79.1.281
PMID:701376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2110225/
Abstract

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.

摘要

本文描述了一种使用来自金黄色葡萄球菌的125I标记蛋白A在十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶中定位抗原-抗体复合物的方法。我们使用该方法探究了海胆和衣藻α-及β-微管蛋白之间的抗原交叉反应性；我们还展示了该方法如何检测针对细胞膜的抗血清中的次要抗体种类。

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Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.细胞抗原在十二烷基硫酸钠-聚丙烯酰胺凝胶中的定位
J Cell Biol. 1978 Oct;79(1):281-5. doi: 10.1083/jcb.79.1.281.
2
Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism.微管蛋白α亚基的异质性以及单个生物体中微管蛋白的变异性。
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3
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Actin-like and tubulin-like proteins in synaptic junctional complexes.突触连接复合体中的肌动蛋白样蛋白和微管蛋白样蛋白。
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Microbios. 1984;41(160):87-98.
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[Platelet membrane glycoproteins of thrombocytopenic disorders: analysis using isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis in a two-dimensional technic].
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引用本文的文献

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Activation of adenylyl cyclase in Chlamydomonas reinhardtii by adhesion and by heat.莱茵衣藻中腺苷酸环化酶通过黏附作用和热作用被激活。
J Cell Biol. 1993 Jul;122(1):137-47. doi: 10.1083/jcb.122.1.137.
2
Endogenous cell surface lectin in Dictyostelium: quantitation, elution by sugar, and elicitation by divalent immunoglobulin.盘基网柄菌中的内源性细胞表面凝集素：定量、糖洗脱及二价免疫球蛋白激发
J Cell Biol. 1980 Dec;87(3 Pt 1):682-90. doi: 10.1083/jcb.87.3.682.
3
Experimental dissection of flagellar surface motility in Chlamydomonas.衣藻鞭毛表面运动的实验解剖
J Cell Biol. 1980 Aug;86(2):656-65. doi: 10.1083/jcb.86.2.656.
4
Brush-border alpha-actinin? Comparison of two proteins of the microvillus core with alpha-actinin by two-dimensional peptide mapping.刷状缘α-辅肌动蛋白？通过二维肽图分析比较微绒毛核心的两种蛋白质与α-辅肌动蛋白。
J Cell Biol. 1980 Aug;86(2):466-74. doi: 10.1083/jcb.86.2.466.
5
Immunologic recognition of cell surface antigens in normal mouse neural tissues and in neuroepithelial cells of the OTT-6050 mouse teratoma. A radiometric, gel electrophoretic and morphologic (immunofluorescence and immunoperoxidase) study.正常小鼠神经组织及OTT-6050小鼠畸胎瘤神经上皮细胞中细胞表面抗原的免疫识别。一项放射性测定、凝胶电泳及形态学（免疫荧光和免疫过氧化物酶）研究。
Acta Neuropathol. 1982;56(3):214-24. doi: 10.1007/BF00690638.
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Immunofluorescence localization at the mammalian neuromuscular junction of the Mr 43,000 protein of Torpedo postsynaptic membranes.电鳐突触后膜43000道尔顿蛋白质在哺乳动物神经肌肉接头处的免疫荧光定位。
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Relation between cell activity and the distribution of cytoplasmic actin and myosin.细胞活性与细胞质肌动蛋白和肌球蛋白分布之间的关系。
J Cell Biol. 1981 Jul;90(1):84-91. doi: 10.1083/jcb.90.1.84.
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The cytoskeleton of digitonin-treated rat hepatocytes.洋地黄皂苷处理的大鼠肝细胞的细胞骨架
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Immunocytochemical localization of coated vesicle protein in rodent nervous system.啮齿动物神经系统中被膜小泡蛋白的免疫细胞化学定位
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本文引用的文献

1
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
2
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
3
Phylogenetic insight into evolution of mammalian Fc fragment of gamma G globulin using staphylococcal protein A.利用葡萄球菌蛋白A对哺乳动物γG球蛋白Fc片段的进化进行系统发育分析。
J Immunol. 1970 Jan;104(1):140-7.
4
Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.衣藻鞭毛。I. 微管、基质、膜和茸鞭的分离及电泳分析。
J Cell Biol. 1972 Sep;54(3):507-39. doi: 10.1083/jcb.54.3.507.
5
A comparison of membrane proteins of normal and transformed cells by lactoperoxidase labeling.通过乳过氧化物酶标记法对正常细胞和转化细胞的膜蛋白进行比较。
Proc Natl Acad Sci U S A. 1974 Feb;71(2):489-92. doi: 10.1073/pnas.71.2.489.
6
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.人红细胞膜主要多肽的电泳分析。
Biochemistry. 1971 Jun 22;10(13):2606-17. doi: 10.1021/bi00789a030.
7
Flagellar doublet microtubules: fractionation of minor components and alpha-tubulin from specific regions of the A-tubule.鞭毛双联体微管：从小管A的特定区域分离次要成分和α-微管蛋白。
J Cell Sci. 1976 Mar;20(2):405-39. doi: 10.1242/jcs.20.2.405.
8
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.用葡萄球菌蛋白A-抗体吸附剂从细胞中快速分离抗原：抗体-抗原复合物与蛋白A相互作用的参数
J Immunol. 1975 Dec;115(6):1617-24.
9
A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue G250.一种使用考马斯亮蓝G250对聚丙烯酰胺凝胶中的蛋白质进行染色的新技术。
Anal Biochem. 1977 Oct;82(2):580-2. doi: 10.1016/0003-2697(77)90197-x.