Busse W W, Anderson C L, Cooper W
J Allergy Clin Immunol. 1981 Mar;67(3):178-84. doi: 10.1016/0091-6749(81)90058-0.
Isolated human polymorphonuclear leukocytes (PMNs) release beta glucuronidase (BG) lysosomal enzyme during incubation with complement-activated zymosan particles. In asthma, isoproterenol (ISO) and histamine (HIS) inhibition of PMN lysosomal enzyme release is impaired while the prostaglandin E1 (PGE1) response is normal. During a respiratory infection provoking asthma, the PMN response to ISO is further impaired. The PMN response to ISO, HIS, and PGE1 is similarly impaired following an in vitro incubation with a live influenza bivalent (A + B) vaccine. In the following study, granulocytes were isolated from normal (n = 10) and asthma patients (n = 29). The isolated cells were incubated with the bivalent influenza vaccine (1 PMN:1 egg infective dose50) for 30 min. Following this in vitro virus incubation, granulocytes from normal and asthma patients had an imparied response to ISO, HIS, and PGE1. Granulocytes from asthma patients having a history of wheezing with respiratory illnesses did not have a greater impairment in the agonist response following virus incubation than normals or allergic asthma patients. However, in asthma patients (n = 6) receiving prednisone (10 to 20 mg/day), the in vitro vaccine incubation did not impair the PMN ISO response. A similar protective effect was found if granulocytes were incubated with hydrocortisone (1,4 X 10(-4) M) prior to exposure to the influenza vaccine.
分离出的人多形核白细胞（PMN）在与补体激活的酵母聚糖颗粒孵育期间会释放β-葡萄糖醛酸酶（BG）溶酶体酶。在哮喘中，异丙肾上腺素（ISO）和组胺（HIS）对PMN溶酶体酶释放的抑制作用受损，而前列腺素E1（PGE1）反应正常。在引发哮喘的呼吸道感染期间，PMN对ISO的反应进一步受损。在与活的二价流感（A+B）疫苗进行体外孵育后，PMN对ISO、HIS和PGE1的反应同样受损。在以下研究中，从正常（n = 10）和哮喘患者（n = 29）中分离出粒细胞。将分离出的细胞与二价流感疫苗（1个PMN：1个鸡蛋感染剂量50）孵育30分钟。在这种体外病毒孵育后，正常和哮喘患者的粒细胞对ISO、HIS和PGE1的反应受损。有呼吸道疾病喘息病史的哮喘患者的粒细胞在病毒孵育后对激动剂的反应受损程度并不比正常人或过敏性哮喘患者更大。然而，在接受泼尼松（10至20毫克/天）的哮喘患者（n = 6）中，体外疫苗孵育并未损害PMN对ISO的反应。如果在接触流感疫苗之前将粒细胞与氢化可的松（1.4×10^(-4) M）孵育，也会发现类似的保护作用。
