Busse W W, Cooper W, Warshauer D M, Dick E C, Wallow I H, Albrecht R
Am Rev Respir Dis. 1979 Apr;119(4):561-9. doi: 10.1164/arrd.1979.119.4.561.
The release of the lysosomal enzyme beta-glucuronidase from granulocytes follows incubation in vitro with complement-activated zymosan particles. Release of beta-glucuronidase is inhibited by isoproterenol, histamine, and prostaglandin E1. This in vitro model was used to study the effect of incubating a live, bivalent (A + B) influenza vaccine on the granulocyte response to the agonists described. After incubation in vitro with the live, bivalent influenza vaccine, there was a significantly impaired granulocyte reponse to all 3 agonists. The change in the response of polymorphonuclear leukocytes to isoproterenol was similar to an impairment in beta-adrenergic response found during respiratory infections in vivo. The viral-induced changes in the granulocyte response to isoproterenol may reflect similar alteration in other tissues, such as variable control of the airways and provide one explanation for the occurrence of airway dysfunction during respiratory infections.
在体外与补体激活的酵母聚糖颗粒一起孵育后，粒细胞会释放溶酶体酶β-葡萄糖醛酸酶。异丙肾上腺素、组胺和前列腺素E1可抑制β-葡萄糖醛酸酶的释放。该体外模型用于研究活的二价（A+B）流感疫苗孵育对粒细胞对所述激动剂反应的影响。在体外与活的二价流感疫苗孵育后，粒细胞对所有3种激动剂的反应均显著受损。多形核白细胞对异丙肾上腺素反应的变化类似于在体内呼吸道感染期间发现的β-肾上腺素能反应受损。病毒诱导的粒细胞对异丙肾上腺素反应的变化可能反映了其他组织中的类似改变，如气道的可变控制，并为呼吸道感染期间气道功能障碍的发生提供了一种解释。
