通过对高分子量组分中的免疫球蛋白进行放射免疫测定来测量循环免疫复合物。
Measurement of circulating immune complexes by radioimmunoassay of immunoglobulin in high molecular weight fractions.
作者信息
Kilpatrick D C, Yap P L, Irvine W J
出版信息
J Immunol Methods. 1980;39(3):269-75. doi: 10.1016/0022-1759(80)90061-7.
A method for detecting circulating immune complexes is described based on radioimmunoassay of IgG following the rapid separation of immune complexes from monomeric IgG on short columns of Sephacryl S-300. Values obtained using sera from patients with immune complex associated diseases were distinctly higher than those obtained with sera from healthy control subjects. The same serum samples were assayed by 3 other methods for detecting immune complexes; significant correlation was obtained.
描述了一种检测循环免疫复合物的方法，该方法基于在Sephacryl S - 300短柱上快速从单体IgG中分离免疫复合物后对IgG进行放射免疫测定。使用免疫复合物相关疾病患者血清获得的值明显高于使用健康对照受试者血清获得的值。用其他3种检测免疫复合物的方法对相同的血清样本进行了检测；结果获得了显著相关性。
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