Wager O, Lindström P, Räsänen J A, Kekomäki R, Ziola B, Salmi A, Isomäki H, Skrifvars B, Penttinen K
Clin Exp Immunol. 1981 Oct;46(1):149-60.
Six tests for circulating immune complexes (CIC) developed in four laboratories and representing four main principles [affinity of human platelets, Clq, of RF for aggregated IgG, and of conglutinin (Kg) for complex-bound C3] were evaluated on series of SLE and definite RA. All tests detected human model complexes in the presence of NHS and discriminated the patient series from the blood donor series, most powerful being the PIPA (platelet test). The high correlation between the RF-binding inhibition tests (RFbI) and the RF-latex test suggested interference due to intrinsic RFs. This received further support from experimental analyses in which RA sera mixed with varying doses of heat-aggregated IgG were assayed by the pRFbI test.
对在四个实验室开发的、代表四种主要原理（人血小板的亲和力、Clq、RF 对聚集 IgG 的亲和力以及胶固素（Kg）对复合物结合 C3 的亲和力）的六种循环免疫复合物（CIC）检测方法，在系统性红斑狼疮（SLE）和确诊类风湿关节炎（RA）系列患者中进行了评估。所有检测方法在存在正常人类血清（NHS）的情况下均能检测到人类模型复合物，并能将患者系列与献血者系列区分开来，其中最有效的是血小板免疫粘附试验（PIPA）。RF 结合抑制试验（RFbI）和 RF 乳胶试验之间的高度相关性表明存在内源性 RF 导致的干扰。这一观点在实验分析中得到了进一步支持，在该实验分析中，通过 pRFbI 试验对与不同剂量热聚集 IgG 混合的 RA 血清进行了检测。
