Nilsson B O, Naeslund G, Curman B
J Exp Zool. 1980 Nov;214(2):177-80. doi: 10.1002/jez.1402140208.
Mouse blastocysts in delay of implantation and after an 18-hour activation for implantation by estrogen were recovered by flushing with glutaraldehyde containing Alcian Blue or by flushing with cold Dulbecco's PBS containing 0.1% sodium azide for further processing according to an Alcian Blue technique and a ConA-latex technique, respectively. Blastocysts in delay of implantation showed no or only faint staining with Alcian Blue, while blastocysts activated for 18 hours displayed a marked staining of the abembryonic pole. Binding of ConA-latex spheres demonstrated a markedly higher density at the abembryonic end of both delayed and implanting blastocysts. It is concluded that, as demonstrated by the Alcian Blue technique, the abembryonic trophoblast, which is the first one to attach and invade at implantation, has changed the properties of the extracellular coat, probably in a way that favors increase of adhesiveness and invasiveness. The similarity in pattern of ConA binding of both delayed and implanting blastocysts, however, suggests that this property is related more to preimplantational differences in proliferative activity of the two poles than to implantatory changes.
通过用含有阿尔辛蓝的戊二醛冲洗或用含有0.1%叠氮化钠的冷杜尔贝科磷酸盐缓冲盐水（Dulbecco's PBS）冲洗，分别根据阿尔辛蓝技术和刀豆球蛋白A-乳胶技术回收延迟着床的小鼠囊胚以及经雌激素激活18小时以进行着床的小鼠囊胚，以便进一步处理。延迟着床的囊胚用阿尔辛蓝染色无或仅有微弱染色，而激活18小时的囊胚在胚外极显示明显染色。刀豆球蛋白A-乳胶球的结合显示，延迟着床和着床囊胚的胚外端密度明显更高。结论是，如阿尔辛蓝技术所示，着床时最先附着和侵入的胚外滋养层改变了细胞外被的性质，可能是以有利于增加黏附性和侵袭性的方式。然而，延迟着床和着床囊胚的刀豆球蛋白A结合模式相似，这表明这种特性更多地与两极着床前增殖活性的差异有关，而非与着床变化有关。
