大鼠脑内脂肪酶的研究。

Studies on lipase in rat brain.

作者信息

Mizobuchi M, Shirai K, Matsuoka N, Saito Y, Kumagai A

出版信息

J Neurochem. 1981 Jan;36(1):301-3. doi: 10.1111/j.1471-4159.1981.tb02409.x.

DOI:10.1111/j.1471-4159.1981.tb02409.x

Abstract

Lipase activity was measured in homogenates of rat cerebral hemispheres using radioactive glycerol trioleate emulsified with Triton X-100 as substrate. The labeled oleic acid was separated from the ester with a methanol-chloroform-heptane mixture. Under these assay conditions, the activity showed pH optima at about 5.5 and 7.5. The final products of these lipase activities were suggested to be free fatty acid and glycerol.

摘要

使用用吐温X-100乳化的放射性甘油三油酸酯作为底物，在大鼠大脑半球匀浆中测量脂肪酶活性。用甲醇 - 氯仿 - 庚烷混合物将标记的油酸从酯中分离出来。在这些测定条件下，活性在约5.5和7.5处显示出pH最适值。这些脂肪酶活性的最终产物被认为是游离脂肪酸和甘油。