Natarajan V, Dyck P J, Schmid H H
J Neurochem. 1981 Feb;36(2):413-9. doi: 10.1111/j.1471-4159.1981.tb01609.x.
The composition and metabolism of rat sciatic nerve phospholipids were studied 20 weeks after induction of chronic diabetes by intraperitoneal injection of streptozotocin (50 mg/kg). On a wet weight basis the nerves from the diabetic animals showed a 7% decrease in total phospholipid from that of controls and a relative decrease in phosphatidylinositol. Incubations of isolated sciatic nerves of diabetic rats in a medium containing [33P]orthophosphate gave decreased labeling of phosphatidylinositol and substantial changes in the labeling pattern of phosphatidylinositol phosphate and 4,5-bisphosphate from that of controls. The ratio of label in these polyphosphoinositides decreased from 2.5 for normal nerve to about 1.0 for diabetic nerve within a 2-h incubation period. These metabolic alterations were not observed in acutely diabetic animals 5 days after streptozotocin (100 mg/kg) administration. Because polyphosphoinositides may be involved in the control of membrane permeability during axonal conduction, alterations in their relative amounts or turnover rates could be related to the physiological changes of early diabetic neuropathy.
通过腹腔注射链脲佐菌素（50毫克/千克）诱导大鼠慢性糖尿病20周后，对其坐骨神经磷脂的组成和代谢进行了研究。以湿重计，糖尿病动物的神经总磷脂比对照组减少了7%，磷脂酰肌醇相对减少。将糖尿病大鼠的离体坐骨神经在含有[33P]正磷酸盐的培养基中孵育，结果显示磷脂酰肌醇的标记减少，且磷脂酰肌醇磷酸和4,5-二磷酸的标记模式与对照组相比有显著变化。在2小时的孵育期内，这些多磷酸肌醇中的标记物比率从正常神经的2.5降至糖尿病神经的约1.0。在注射链脲佐菌素（100毫克/千克）5天后的急性糖尿病动物中未观察到这些代谢改变。由于多磷酸肌醇可能参与轴突传导过程中膜通透性的控制，其相对含量或周转率的改变可能与早期糖尿病神经病变的生理变化有关。
